Introduction
Autoimmune diseases
Autoantibodies - Introduction
Autoantibodies - Determination
 
Autoantibodies
Rheumatoid Factor
Antinuclear Antibodies (ANA)
Specific Antibodies
Anti-neutrophil Cytoplasmic Antibodies
(ANCA)
Anti-phospholipid Antibodies
Anti-mitochondrial Antibodies (AMA)
Anti-endothelial Cell Antibodies (AECA)
Anti CCP antibodies
Antibodies against DNases
 
Quality Assurance
 
Reference ranges
 
Algorithm
ANA and incidence of diseases
Proposed stepwise diagnosis scheme
Positive Immunoflourescence -
Nucleoplasmic
Positive Immunoflourescence - Nucleolar
Positive Immunoflourescence -
Cytoplasmic
Type of autoimmune diseases
Conditions associated with antinuclear
antibodies (ANA)
 
Slide show
 
References
 
Collaborators
Specific Antibodies
Antiribonucleoprotein and anti-Sm
Antibodies to SS-A/Ro and SS-B/La
Anticentromere (ACA) and anti Scl-70 antibodies
Antibodies to anti-proliferating cell nuclear antigen (PCNA)
Antibodies to nuclear enzymes
PM-1 in polymyositis
DNA antibodies
Antibodies to histones
 
DNA antibodies - Clinical significance

1) Physiologic changes in the concentration of DNA antibodies

2) Pathologic changes in the concentration DNA antibodies
a) Increased DNA antibodies:
1. Anti-ds DNA antibodies:
SLE
Dermatomyositis/Polymyositis
Mixed Connective Tissue Diseases
Rheumatoid Arthritis
Juvenile Rheumatoid Arthritis
2. Anti-ss DNA antibodies
SLE
Progressive Systemic Sclerosis
Rheumatoid Arthritis
Fetty’s Syndrome
Juvenile Rheumatoid Arthritis
Inflammation in general

anti ds-DNA


Reference values
dependent on method

Immunofluorescence method.

negative

ELISA

 

 

Units

negative

< 40

U/ml

equivocal

40 – 60

U/ml

positive

> 60

U/ml


anti ss-DNA


Reference values
dependent on method

Immunofluorescence method.

negative

ELISA

 

 

Units

negative

< 8

U/ml

equivocal

8 – 10

U/ml

positive

> 10

U/ml