Introduction
Autoimmune diseases
Autoantibodies - Introduction
Autoantibodies - Determination
 
Autoantibodies
Rheumatoid Factor
Antinuclear Antibodies (ANA)
Specific Antibodies
Anti-neutrophil Cytoplasmic Antibodies
(ANCA)
Anti-phospholipid Antibodies
Anti-mitochondrial Antibodies (AMA)
Anti-endothelial Cell Antibodies (AECA)
Anti CCP antibodies
Antibodies against DNases
 
Quality Assurance
 
Reference ranges
 
Algorithm
ANA and incidence of diseases
Proposed stepwise diagnosis scheme
Positive Immunoflourescence -
Nucleoplasmic
Positive Immunoflourescence - Nucleolar
Positive Immunoflourescence -
Cytoplasmic
Type of autoimmune diseases
Conditions associated with antinuclear
antibodies (ANA)
 
Slide show
 
References
 
Collaborators
Specific Antibodies
Antiribonucleoprotein and anti-Sm
Antibodies to SS-A/Ro and SS-B/La
Anticentromere (ACA) and anti Scl-70 antibodies
Antibodies to anti-proliferating cell nuclear antigen (PCNA)
Antibodies to nuclear enzymes
PM-1 in polymyositis
DNA antibodies
Antibodies to histones
 
Anticentromere (ACA) and anti Scl-70 antibodies - Determination

Anticentromere (ACA) and anti Scl-70 antibodies - Sample
Serum
Specimen stable at 2-8°C for 1 week.

Anticentromere (ACA) and anti Scl-70 antibodies - Methods

Anticentromere (ACA) and anti Scl-70 antibodies - Indirect immunofluorescence assay
See Antinuclear Antibodies – Indirect immunofluorescence assay.

Anticentromere (ACA) and anti Scl-70 antibodies - Radioimmunoasay
See Appendix.

Anticentromere (ACA) and anti Scl-70 antibodies - ELISA
See Antinuclear Antibodies - ELISA

Material
Pattern: SCL-70
Substrate: Hep2-cells

Description
Fine fluorescence of nuclei, granular decoration of nucleoli, chromosomes positive

Method
Indirect immunoflourescence method.