Methods |
Concentration |
Ouchterlony |
20 - 40 |
µg |
RID |
5 - 10 |
µg |
Immunoelectrophoresis |
20 - 80 |
µg |
“Rocket” immunoelectrophoresis |
2 - 5 |
µg |
Immunonefelometry |
20 - 50 |
µg |
Hemaglutination |
10 - 1000 |
ng |
Complement fixation |
100 - 1000 |
ng |
Immunofluorescence |
1 - 100 |
ng |
Immunoblot |
0.2 - 100 |
pg |
ELISA |
0.1 - 100 |
pg |
Luminoescence |
0.1 - 100 |
pg |
RIA |
0.1 - 100 |
pg |
Table 4 Comparison of the Routine Methods
Method |
Advantage/Disadvantage |
Immunofluorescence |
Native antigens in situ |
Immunodiffusion in gels |
Native antigens in solution |
ELISA, RIA, Hemagglutination |
Purified antigens |
Immunoblot |
Denatured Antigens after electrophoretic separation |
|
(ANCA)
