Introduction
Autoimmune diseases
Autoantibodies - Introduction
Autoantibodies - Determination
 
Autoantibodies
Rheumatoid Factor
Antinuclear Antibodies (ANA)
Specific Antibodies
Anti-neutrophil Cytoplasmic Antibodies
(ANCA)
Anti-phospholipid Antibodies
Anti-mitochondrial Antibodies (AMA)
Anti-endothelial Cell Antibodies (AECA)
Anti CCP antibodies
Antibodies against DNases
 
Quality Assurance
 
Reference ranges
 
Algorithm
ANA and incidence of diseases
Proposed stepwise diagnosis scheme
Positive Immunoflourescence -
Nucleoplasmic
Positive Immunoflourescence - Nucleolar
Positive Immunoflourescence -
Cytoplasmic
Type of autoimmune diseases
Conditions associated with antinuclear
antibodies (ANA)
 
Slide show
 
References
 
Collaborators
Anti-neutrophil Cytoplasmic Antibodies (ANCA)
Cytoplasmic/classical anti-neutrophil cytoplasmic antibodies (c-ANCA)
Perinuclear anti-neutrophil cytoplasmic antibodies (p-ANCA)
 
Perinuclear anti-neutrophil cytoplasmic antibodies (p-ANCA) - Significance

1) Physiologic changes in the concentration of p-ANCA

2) Pathologic changes in the concentration p-ANCA

a) Increased p-ANCA in:
Microscopic polyarteritis
Polyangiitis
Panarteritis nodosa
Chronic polyarthritis

Churg-Strauss syndrome
Goodpasture’s syndrome
Hydralazine-induced lupus erythematosus
Myelitis

Irritable bowel syndrome
Primary sclerosing cholangitis

Reference values
dependent on method

Immunofluorescence method.

negative

ELISA

 

 

Units

negative

< 5

U/ml

positive

> 5

U/ml


Material
Pattern: pANCA
Substrate: Formalin fixed

Description

Evenly distributed granular fluorescence in the cytoplasm

Method
Indirect immunoflourescence method.

pANCA (perinuclear ANCA) is more heterogeneous. In may be directed against myeloperoxidase (as seen most typically but not exclusively in microscopic polyangiitis), lactoferrin,elastese, cathepsin G and other  antigens.
pANCA are defined as antibodies staining the perinuclear cytoplasm or the nucleus.