Introduction
Autoimmune diseases
Autoantibodies - Introduction
Autoantibodies - Determination
 
Autoantibodies
Rheumatoid Factor
Antinuclear Antibodies (ANA)
Specific Antibodies
Anti-neutrophil Cytoplasmic Antibodies
(ANCA)
Anti-phospholipid Antibodies
Anti-mitochondrial Antibodies (AMA)
Anti-endothelial Cell Antibodies (AECA)
Anti CCP antibodies
Antibodies against DNases
 
Quality Assurance
 
Reference ranges
 
Algorithm
ANA and incidence of diseases
Proposed stepwise diagnosis scheme
Positive Immunoflourescence -
Nucleoplasmic
Positive Immunoflourescence - Nucleolar
Positive Immunoflourescence -
Cytoplasmic
Type of autoimmune diseases
Conditions associated with antinuclear
antibodies (ANA)
 
Slide show
 
References
 
Collaborators
Anti-neutrophil Cytoplasmic Antibodies (ANCA)
Cytoplasmic/classical anti-neutrophil cytoplasmic antibodies (c-ANCA)
Perinuclear anti-neutrophil cytoplasmic antibodies (p-ANCA)
 

Cytoplasmic/classical anti-neutrophil cytoplasmic antibodies (c-ANCA) - Clinical significance

1) Physiologic changes in the concentration of c-ANCA

2) Pathologic changes in the concentration c-ANCA
a) Increased c-ANCA in:
Wegener’s granulomatosis
Microscopic polyarteritis
Churg-Strauss syndrome
Tolosa-Hunt syndrome
Polyneuritis cranialis
Peripheral neuropathy
Secondary polychondritis
Facial paralysis
in hemodialysis ptients with renal failure of unknown cause

Reference values
dependent on method

Immunofluorescence method.

negative

ELISA

 

 

Units

negative

0 - 20

U/ml

equivocal

21 – 30

U/ml

positive

> 30

U/ml


Material
Pattern: cANCA
Substrate: Formalin fixed

Description
Similar to pattern on ethanol granulocytes

Method
Indirect immunoflourescence method.

cANCA (cytoplasmic ANCA) is most commonly directed against the serine protease, proteinase 3 and strongly associated with Wegener’s granulomatosis.
cANCA are defined as antibodies staining cytoplasmic granules, usually with accentuated intensity around the nucleus.