Significance Of Cytochemical Reactions

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SIGNIFICANCE OF CYTOCHEMICAL REACTIONS
MYELOPEROXIDASE
ALKALINE PHOSPHATASE
ALPHA-NAPHTHYL-ACETATE-ESTERASE
ACID PHOSPHATASE
PERIODIC-ACID-SCHIFF (PAS) STAIN
SUDAN BLACK B STAIN
 

SUDAN BLACK B STAIN

 


This stain is specific for lipids. Sudan black B stains a variety of lipids, including neutral fat, phospholipids, and sterols.
Normal granulocyte precursors show increased sudanophilia that corresponds roughly to the number of granules. Promyelocytes contain a few sudanophilic granules, and mature polymorphonuclear neutrophils contain large numbers of sudanophilic granules. Eosinophil granules are also sudanophilic, especially on the edge. Monocytes may be unstained or may contain a few discrete sudanophilic granules. Macrophages often contain sudanophilic material.
In general, Sudan black B stain is similar to the peroxidase reaction in distinguishing acute lymphocytic leukemia from acute myelogenous leukemia.