The layer of the cytoplasm surrounding the nucleus increases, and nucleocytoplasmatic ratio decreases (15:1). The cytoplasm is stained pink and contains numerous granules. It is neutrophilic, eosinophilic, and basophilic.
- The cytoplasm of neutrophilic metamyelocytes is colorless or slightly acidophilic and contains secondary neutrophilic granules.
- The cytoplasm of eosinophilic metamyelocytes is slightly acidophilic and contains secondary eosinophilic granules.
- The cytoplasm of basophilic metamyelocytes contains characteristic basophilic granules.